Cell segmentation and pipette identification for automated patch clamp recording
نویسندگان
چکیده
منابع مشابه
One-channel cell-attached patch-clamp recording.
Ion channel proteins are universal devices for fast communication across biological membranes. The temporal signature of the ionic flux they generate depends on properties intrinsic to each channel protein as well as the mechanism by which it is generated and controlled and represents an important area of current research. Information about the operational dynamics of ion channel proteins can b...
متن کاملPatch clamp techniques for single channel and whole-cell recording
The patch clamp technique was first used by Neher and Sakmann (1976) to resolve currents through single acetylcholine-activated channels in cell-attached patches of membrane of frog skeletal muscle. The method they used (described by Neher, Sakmann & Steinbach, 1978) and subsequent refinements (Hamill, Marty, Neher, Sakmann & Sigworth, 1981) have led to techniques for high resolution recording ...
متن کاملMicrochip amplifier for in vitro, in vivo, and automated whole cell patch-clamp recording.
Patch clamping is a gold-standard electrophysiology technique that has the temporal resolution and signal-to-noise ratio capable of reporting single ion channel currents, as well as electrical activity of excitable single cells. Despite its usefulness and decades of development, the amplifiers required for patch clamping are expensive and bulky. This has limited the scalability and throughput o...
متن کاملInnovative Methodology Microchip amplifier for in vitro, in vivo, and automated whole cell patch-clamp recording
Reid R. Harrison, Ilya Kolb, Suhasa B. Kodandaramaiah, Alexander A. Chubykin, Aimei Yang, Mark F. Bear, Edward S. Boyden,* and Craig R. Forest* Intan Technologies, Los Angeles, California; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia; Media Lab, Massachusetts Institute of Technology, Cambridge, Massachusetts; McGovern Institute, Mass...
متن کاملAutomated planar patch-clamp.
Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as controlling many metabolic functions. Furthermore, i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Robotics and Biomimetics
سال: 2014
ISSN: 2197-3768
DOI: 10.1186/s40638-014-0020-5